Expression of Tn5-derived kanamycin resistance in the fungus Phycomyces blakesleeanus |
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Authors: | José Arnau, Francisco J. Murillo Santiago Torres-Martí nez |
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Affiliation: | (1) Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, E-30071 Murcia, Spain |
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Abstract: | Summary A plasmid, carrying the Tn5 gene for kanamycin resistance lacking its own promoter, has successfully been used in the selection of DNA sequences of the fungus Phycomyces blakesleeanus having promoter activity in Escherichia coli. Many of these sequences were also effective in promoting resistance to kanamycin when the corresponding chimeric plasmids were introduced in the fungus via spheroplast transformation. The selected phenotype was easily propagated through vegetative spores and behaved as a stable character since it was not appreciably lost in the absence of selection. |
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Keywords: | Phycomyces Transposon Tn5 Kanamycin Fungal transformation Fungal promoters |
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