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Resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene |
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| Authors: | Sivamani Elumalai Brey Christopher W. Talbert Luther E. Young Mark A. Dyer William E. Kaniewski Wojciech K. Qu Rongda |
| Affiliation: | (1) Department of Plant Sciences, Montana State University, Bozeman, MT 59717-3140, USA;(2) MAHYCO – Life Sciences Research Centre, PO box 76, Jalna, Maharashtra, India;(3) Department of Entomology, Rutgers University, New Brunswick, NJ 08903, USA;(4) Monsanto Life Sciences Company, 700 Chesterfield Pkwy, St. Louis, MO 63198, USA;(5) Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA |
| Abstract: | Wheat (Triticum aestivum) plants were stably transformed with the coat protein (CP) gene of wheat streak mosaic virus (WSMV) by the biolistic method. Eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to WSMV. One line showed high resistance to inoculations of two WSMV strains. This line had milder symptoms and lower virus titer than control plants after inoculation. After infection, new growth did not show symptoms. The observed resistance was similar to the  recovery type resistance described previously using WSMV NIb transgene and in other systems. This line looked morphologically normal but had an unusually high transgene copy number (approximately 90 copies per 2C homozygous genome). Northern hybridization analysis indicated a high level of degraded CP mRNA expression. However, no coat protein expression was detected. |
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