Changes in the activities of some enzymes of carbohydrate metabolism in Amaryllis vittata pollen suspension cultures

Amaryllis vittata pollen grown in suspension cultures utilizeexogenous carbohydrates for starch synthesis and pollen tubegrowth; tube growth is influenced by both monosaccharides andoligosaccharides in the culture medium but not by ribose andxylose. The level of starch in germinating pollen was influencedby the availability of exogenous carbohydrates. Specific activitiesof some enzymes of glycolysis and pentose phosphate pathwaysvaried during pollen germination. The activities of all theenzymes, except glucose-6-phosphate dehydrogenase, showed moreor less pronounced increase initially, followed by a generaldecrease in later stages. The increase in the enzyme activitieswas affected by the presence of cycloheximide in the incubationmedium. (Received October 20, 1977; )