Clavulanic acid biosynthesis and genetic manipulation for its overproduction |
| |
Authors: | Ju Yeon Song Susan E Jensen Kye Joon Lee |
| |
Institution: | (1) School of Biological Sciences, Seoul National University, Seoul, 151-747, South Korea;(2) Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea;(3) Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9, Canada; |
| |
Abstract: | Clavulanic acid, a β-lactamase inhibitor, is used together with β-lactam antibiotics to create drug mixtures possessing potent
antimicrobial activity. In view of the clinical and industrial importance of clavulanic acid, identification of the clavulanic
acid biosynthetic pathway and the associated gene cluster(s) in the main producer species, Streptomyces clavuligerus, has been an intriguing research question. Clavulanic acid biosynthesis was revealed to involve an interesting mechanism
common to all of the clavam metabolites produced by the organism, but different from that of other β-lactam compounds. Gene
clusters involved in clavulanic acid biosynthesis in S. clavuligerus occupy large regions of nucleotide sequence in three loci of its genome. In this review, clavulanic acid biosynthesis and
the associated gene clusters are discussed, and clavulanic acid improvement through genetic manipulation is explained. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|