Conversion of fat into yeast biomass in protein-containing waste-water |
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Authors: | Stefan Rydin Göran Molin Inge Nilsson |
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Affiliation: | (1) Department of Applied Microbiology, Lund Institute of Technology, Chemical Center, P. O. Box 124, S-22100 Lund, Sweden;(2) Present address: Department of Environmental Technology, Danish Technological Institute, P. O. Box 141, DK-2630 Taastrup, Denmark;(3) Present address: Bioinvent International AB, Ideon, S-22370 Lund, Sweden |
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Abstract: | Summary Candida tropicalis S001 was grown on the lipid fraction of a protein-containing waste-water in order to (i) remove fat from the water, and (ii) produre yeast biomass for feed. The yeast cells were separated from the waste-water by sedimentation. Defatted waste-water was used for methane production and gave a yield of a 0.3 m3 methane/kg reduced chemical oxygen demand. The maximum specific growth rate (µmax) of C. tropicalis growing on waste-water fat at pH 4.0 was 0.35 h–1; the fat content was decreased from 8 g/l to about 0.1 g/l within 24 h. In continous culture a corresponding reduction was maintained at dilution rates up to 0.36 h–1. The effect on growth of pH, temperature and CO2 concentration was studied with triolein as the major carbon source. The µmax was nearly constant (0.16 h–1) in the pH and temperature range of 3.2–4.0 and 30°–38° C, respectively; 10% CO2 was optimal for growth. Growth on triolein resulted in a biomass yield of 0.70 g dry weight/g fat.Offprint requests to: S. Rydin |
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