Cellular localization of the receptor-dependent and receptor-independent uptake of human LDL in the liver of normal and 17 alpha-ethinyl estradiol-treated rats |
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Authors: | L Harkes T J van Berkel |
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Affiliation: | Department of Biochemistry I, Medical Faculty, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands |
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Abstract: | The cellular localization in the liver of the receptor-dependent and -independent uptake of human low density lipoprotein (LDL) in normal and 17 alpha-ethinyl estradiol-treated rats was investigated by the simultaneous in vivo injection of human 131I-LDL and human reductive methylated 125I-LDL. The cells were subsequently isolated by a low temperature method. In untreated rats, after 30 min of in vivo circulation of human LDL, 57% of the receptor-dependent liver-association of human LDL occurs in non-parenchymal cells and 43% in parenchymal cells. Estradiol treatment of rats for 3 days selectively increases the receptor-dependent cell-association of human LDL with hepatocytes (17-fold), while the receptor-dependent cell-association with non-parenchymal cells is not affected. |
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Keywords: | Ethinyl estradiol Low density lipoprotein Non-parenchymal liver cell Parenchymal liver cell Reductive methylated LDL LDL low density lipoprotein Me-LDL reductive methylated LDL |
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