Involvement of blood-group-B-active trisaccharides in Ca2+-dependent cell-cell adhesion in the Xenopus blastula |
| |
Authors: | K H Nomura Ryuji Kobayashi Yoshio Hirabayashi Megumi Fujisue-Sakai Souhei Mizuguchi Kazuya Nomura |
| |
Institution: | (1) Laboratory of Developmental Biology, Department of Biology, Faculty of Science 33, Fukuoka 812, Japan, JP;(2) The Keck Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA, US;(3) Laboratory for Glyco Cell Biology, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), 2-1, Hirosawa, Wako-shi, Saitama, 351-01, Japan, JP |
| |
Abstract: | Despite their wide distribution in various organisms, no physiological roles have been proposed for the human blood-group-ABO
(ABH)-active trisaccharides. Here we show that monoclonal antibodies against human blood-group-B-active trisaccharides (B-substance)
completely block the Ca2+-dependent cell-cell adhesion system of frog (Xenopus
laevis) embryonic cells. Synthetic B-substance or B-active glycopeptides also disrupt the Ca2+ -dependent cell-cell adhesion. These results suggest that blood-group-B-active substances play a role in cell-cell adhesion.
Blood-group-B-active substances were found as glycoproteins and as glycosphingolipids. In order to identify B-active glycoproteins
active in cell-cell adhesion, we purified B-active membrane glycoproteins by two-dimensional electrophoresis and found that
they are 45- to 58-kDa proteins with pI(s) ranging from 4.0 to 5.3. They are glycosylphosphatidyl inositol (GPI) anchored.
Amino acid sequence analysis showed that the purified B-active GPI-anchored proteins are homologues of soluble Xenopus cortical granule lectins (CGL). The results suggest that the B-active membrane glycoproteins are GPI-anchored forms of the
lectin and are directly involved in frog Ca 2+-dependent cell-cell adhesion.
Received: 16 September 1997 / Accepted 19 November 1997 |
| |
Keywords: | Cadherin Amphibian Lectin GPI-anchor ABO blood group |
本文献已被 SpringerLink 等数据库收录! |
|