Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization |
| |
| Institution: | 1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Department of Ecology, University of Kaiserslautern, Kaiserslautern 67653, Germany;1. Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;2. Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;3. Department of Bacteriology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;4. Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic;5. Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno and Studenec, Czech Republic;1. Universität Salzburg, FB Organismische Biologie, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria;2. Inha University, Biological Sciences, 402-251 Incheon, South Korea;3. Universität Kaiserslautern, FB Biologie, Erwin-Schrödingerstrasse 14, D-67663 Kaiserslautern, Germany;1. Department of Animal Parasitology, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;2. Hangzhou Key Laboratory for Animal Sciences and Technology, School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China;3. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, South China Normal University, Guangzhou 510631, China;2. Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;3. Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia;1. Universität Salzburg, FB Organismische Biologie, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria;2. Boise State University, Department of Biological Sciences, Boise, ID 83725-1515, USA;3. University of the West Indies, Electron Microscopy Unit, Kingston, Jamaica;4. Universität Kaiserslautern, FB Biologie, Erwin-Schrödingerstrasse 14, D-67663 Kaiserslautern, Germany;1. Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;2. Department of Biological Sciences, Smith College, Northampton, MA 01063, USA;3. Program in Organismic and Evolutionary Biology, UMass-Amherst, Amherst, MA 01003, USA |
| |
| Abstract: | Many scuticociliatid ciliates are regarded as devastating pathogens in aquaculture. Among these, Pseudocohnilembus persalinus is a facultative pathogen that often results in refractory diseases of mariculture fish. Although traditional silver staining methods have been successfully used to identify these ciliates, their identification is hampered by their small size and their morphological similarity to closely related species. We designed an alternative method of identification of P. persalinus using an SSU-rDNA targeted oligonucleotide probe labeled with a fluorochrome, and optimized in a fluorescence in situ hybridization (FISH) protocol. The assay results in a clear identification by strong fluorescence signals from the oligonucleotide probe. The method can be used for quick and early detection of P. persalinus infections on host fish, as well as other susceptible organisms in aquiculture water. It may also be used in studies of the geographical distribution of this scuticociliate. |
| |
| Keywords: | Molecular methods Oligonucleotide probes Scuticociliates SSU-rDNA |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
