Effects of the root of Platycodon grandiflorum on airway mucin hypersecretion in vivo and platycodin D3 and deapi-platycodin on production and secretion of airway mucin in vitro |
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| Institution: | 1. Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 303-131, Republic of Korea;2. Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea;3. Division of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 136-713, Republic of Korea;1. Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 701-300, Republic of Korea;2. The First Affiliated Hospital of Xinxiang Medical University, Xinxiangshi, Henansheng 453-100, China;1. Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China;2. Harbin Institute of Technology, Harbin 150090, China;1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;2. Department of Family Medicine, China Medical University Hospital, Taichung, Taiwan;3. School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan;4. Department of Nutrition, China Medical University, Taichung, Taiwan;5. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan;6. Department of Nursing, Mei Ho University, Pingguang Road, Pingtung, Taiwan;7. Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan;8. Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;9. Chinese Medicine Department, China Medical University Beigang Hospital, Taichung, Taiwan;10. Department of Biotechnology, Bharathiar University, Coimbatore 641046, India;11. Translation Research Core, China Medical University Hospital, China Medical University, Taichung, Taiwan;12. Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan;13. Department of Biological Science, Asia University, Taichung, Taiwan;14. Faculty of Applied Sciences, Ton Duc Thang University, Tan Phong Ward, District 7, 700000 Ho Chi Minh City, Vietnam |
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| Abstract: | We investigated whether aqueous extract of the root of Platycodon grandiflorum A. de Candolle (APG), platycodinD3 and deapi-platycodin significantly affect the production and secretion of airway mucin using in vivo and in vitro experimental models. Effect of APG was checked on hypersecretion of pulmonary mucin in sulfur dioxide-induced bronchitis in rats. Confluent NCI-H292 cells were pretreated with platycodinD3 or deapi-platycodin for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate) for 24 h. The MUC5AC mucin production and secretion were measured by ELISA. The results were as follows: (1) APG stimulated the secretion of airway mucin in sulfur dioxide-induced bronchitis rat model; (2) platycodinD3 and deapi-platycodin inhibited the production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively; (3) however, platycodinD3 and deapi-platycodin did not inhibit but stimulated the secretion of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively. This result suggests that aqueous extract of P. grandiflorum A. de Candolle and the two natural products derived from it, platycodinD3 and deapi-platycodin, can regulate the production and secretion of airway mucin and, at least in part, explains the traditional use of aqueous extract of P. grandiflorum A. de Candolle as expectorants in diverse inflammatory pulmonary diseases. |
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