Promoting osteoblast differentiation by the flavanes from Huangshan Maofeng tea is linked to a reduction of oxidative stress |
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| Affiliation: | 1. Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China;2. College of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China;3. Analysis Center of Guangdong Medical College, Guangdong Medical College, Zhanjiang 524023, Guangdong, China;4. Shenzhen Xinpeng Shengwu Gongcheng Co. Ltd., Shenzhen 518055, Guangdong, China;1. Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China;2. College of Chemistry and Chemical Engineering, HaiNan Normal University, Hai Kou 571158, China;3. Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China;1. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;2. Department of Physics, Ocean University of China, Qingdao 266100, China;1. Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621999, PR China;2. Key Laboratory of Optoelectronics Technology and System, Ministry of Education, Chongqing University, Chongqing 400044, PR China;1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China;2. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, PR China;1. Laboratoire d''Etude des Matériaux Avancés et Applications (LEM2A), Université Moulay Ismaïl, FSM-FPE-ESTM, BP 11201 Zitoune, Meknes, Morocco;2. Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier, Montpellier F-34095, France;1. University of Opole, Faculty of Chemistry, 48 Oleska Street, 45-052 Opole, Poland;2. Institute of Physical Chemistry, Polish Academy of Sciences, 44/52 Kasprzaka, 01-224 Warsaw, Poland;3. Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nám 2, 166 10 Prague, Czech Republic |
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| Abstract: | Epidemiological evidence has shown an association between tea consumption and the prevention of bone loss in the elderly. Previous studies indicated that green tea exerted osteoprotective effect in vivo. This study aims to investigate the constituents in Huangshan Maofeng tea and systemically evaluate their antioxidative and osteogenic effects in vitro. Five flavanes, isolated from Huangshan Maofeng tea, showed effects on proliferation of osteoblastic cells and ameliorated H2O2-induced C2C12 mouse myoblast cell apoptosis at 3.125–50 μg/ml. (−)-Epicatechin observably increased alkaline phosphatase (ALP) activity and hydroxyproline content. (−)-Epiafzelechin at 25 μg/ml significantly increased the area of mineralized bone nodules. The activities of flavanes in promoting osteblastic proliferation and differentiation are positively correlated with activities in protecting against apoptosis in C2C12 cells. It indicates that anti-osteoporosis effect of these flavanes may be linked to their antioxidative activity. The observed effects of these flavanes suggest that these flavanes may have beneficial effects on bone health. |
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| Keywords: | Flavane Osteoporosis Oxidative stress Green tea |
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