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Stephanoeca arndti spec. nov. – First cultivation success including molecular and autecological data from a freshwater acanthoecid choanoflagellate from Samoa
Institution:1. Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-1, SK-842 15 Bratislava, Slovak Republic;2. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 23 Bratislava, Slovak Republic;3. FB Organismal Biology, Faculty of Natural Sciences, Salzburg University, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria;1. Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States;2. Biomedical Laboratory Diagnostics, Michigan State University, East Lansing, MI 48824, United States;3. Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States;4. Faculty of Pharmacy, Universidade Federal do Minas Gerais, Belo Horizonte, Brazil;1. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, United States;2. Department of Medicine, University of California, San Diego, La Jolla, CA 92093, United States;3. Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, United States;4. Department and Pharmacology, University of California, San Diego, La Jolla, CA 92093, United States
Abstract:Until recently acanthoecid choanoflagellates have been described only from marine and brackish waters. Here I describe a distinct, strictly freshwater acanthoecid species from Samoa based on its morphology, ecology and molecular biological data (partial Small Subunit rDNA). The lorica of the species is characterised by two extensions at the posterior chamber which are used for attachment to the substratum. The posterior chamber is constructed of irregularly arranged costae. The anterior chamber consists of four transverse costal rings and 14–18 longitudinal costae. Despite its sturdy appearance, the lorica was extremely sensitive to water turbulence and movements of the water. The species showed a salinity tolerance of 0.5 practical salinity units with reduced growth rates and a temperature tolerance range of 20–34 °C. According to the morphology, phylogenetic analysis, and autecology of the species it was classified as a member of the genus Stephanoeca.
Keywords:Autecology  Choanoflagellida  Freshwater acanthoecid  Phylogeny  Stephanoeca  Taxonomy
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