Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide |
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| Institution: | 1. Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran;2. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran;3. The Student Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran;4. Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran;1. Institute of Nano Science and Nano Technology, University of Kashan, P.O. Box. 87317-51167, Kashan, Iran;2. Young Researchers and Elites Club, Kashan Branch, Islamic Azad University, Kashan, Iran;1. Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic;2. Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic;3. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic;1. Actinobacterial Research Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India;2. Center for Nanoscience and Nanotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India;1. Glyn O. Phillips Hydrocolloid Research Centre at HUT, School of Food and Pharmaceutical Engineering, Faculty of Light Industry, Hubei University of Technology, Wuhan 430068, China;2. Department of Chemistry, Glyndwr University, Plas Coch, Mold Road, Wrexham LL11 2AW, UK;3. Glyn O. Phillips Hydrocolloid Research Center, Glyndwr University, Plas Coch, Mold Road, Wrexham LL11 2AW, UK |
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| Abstract: | The present study was designed to evaluate antioxidant and cytotoxic effect of selenium nanoparticles (Se NPs) biosynthesized by a newly isolated marine bacterial strain Bacillus sp. MSh-1. An organic–aqueous partitioning system was applied for purification of the biogenic Se NPs and the purified Se NPs were then investigated for antioxidant activity using DPPH scavenging activity and reducing power assay. Cytotoxic effect of the biogenic Se NPs and selenium dioxide (SeO2) on MCF-7 cell line was assesed by MTT assay. Tranmission electron micrograph (TEM) of the purified Se NPs showed individual and spherical nanostructure in size range of about 80–220 nm. The obtained results showed that, at the same concentration of 200 μg/mL, Se NPs and SeO2 represented scavenging activity of 23.1 ± 3.4% and 13.2 ± 3.1%, respectively. However, the data obtained from reducing power assay revealed higher electron-donating activity of SeO2 compared to Se NPs. Higher IC50 of the Se NPs (41.5 ± 0.9 μg/mL) compared to SeO2 (6.7 ± 0.8 μg/mL) confirmed lower cytotoxicity of the biogenic Se NPs on MCF-7 cell line. |
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| Keywords: | Biogenic selenium nanoparticles Antioxidant Selenium dioxide MCF-7 cell line |
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