Support for the coevolution of Neoparamoeba and their endosymbionts,Perkinsela amoebae-like organisms |
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| Institution: | 1. National Centre for Mari^#^ne Conservation and Resource Sustainability, University of Tasmania, Locked Bag 1370, Launceston, Tasmania, Australia;2. Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia;3. Faculty of Science, Masaryk University, Kotlářská 2, Brno, Czech Republic;4. Department of Bioinformatics, University of Würzburg, Am Hubland, 97074 Würzburg, Germany;5. Fish Health Unit, Department of Primary Industries, Parks, Water & Environment, 165 Westbury Road, Prospect, Tasmania, Australia;1. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity & College of Fisheries, Ocean University of China, Qingdao 266003, China;2. Faculty of Fisheries, Nagasaki University, Bunkyo machi 1-14, Nagasaki 852-8521, Japan;1. Zoological Survey of India, Prani Vigyan Bhawan, M-Block, New Alipore, Kolkata 700 053, India;2. School of Bioscience and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy;1. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;2. The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China;3. Department of Life Sciences, Natural History Museum, London SW7 5BD, UK;1. Department of Biological Science, College of Natural Sciences, University of Ulsan, Ulsan 680-749, South Korea;2. Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-1, 842 15 Bratislava, Slovak Republic;1. NCMCRS, Locked Bag 1370, University of Tasmania, Launceston, Tas 7250, Australia;2. CSIRO Marine and Atmospheric Research, QBP, 306 Carmody Rd., St. Lucia, Qld 4067, Australia |
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| Abstract: | Some of the species from the genus Neoparamoeba, for example N. perurans have been shown to be pathogenic to aquatic animals and thus have economic significance. They all contain endosymbiont, Perkinsela amoebae like organisms (PLOs). In this study we investigated phylogenetic ambiguities within the Neoparamoeba taxonomy and phylogenetic congruence between PLOs and their host Neoparamoeba to confirm the existence of a single ancient infection/colonisation that led to cospeciation between all PLOs and their host Neoparamoeba. DNA was extracted and rRNA genes from host amoeba and endosymbiont were amplified using PCR. Uncertainties in the Neoparamoeba phylogeny were initially resolved by a secondary phylogenetic marker, the internal transcribed spacer 2 (ITS2). The secondary structure of ITS2 was reconstructed for Neoparamoeba. The ITS2 was phylogenetically informative, separating N. pemaquidensis and N. aestuarina into distinct monophyletic clades and designating N. perurans as the most phylogenetically divergent Neoparamoeba species. The new phylogenetic data were used to verify the tree topologies used in cophylogenetic analyses that revealed strict phylogenetic congruence between endosymbiotic PLOs with their host Neoparamoeba. Strict congruence in the phylogeny of all PLOs and their host Neoparamoeba was demonstrated implying that PLOs are transmitted vertically from parent to daughter cell. |
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| Keywords: | Endosymbiont ITS2 Phylogeny |
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