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Antileishmanial activity of essential oil from Chenopodium ambrosioides and its main components against experimental cutaneous leishmaniasis in BALB/c mice
Institution:1. Parasitology Department, Institute of Tropical Medicine “Pedro Kouri”, Havana, Cuba;2. Department of Chemistry, Institute of Pharmacy and Food, Havana University, Cuba;3. Biochemical Pharmacology and Toxicology Unit, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria;1. Medical Malacology and Parasitology Departments, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt;2. Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt;1. Laboratory of Biotechnology, Protection and Valorization of Plant Resources, Phytochemistry and Pharmacology of Medicinal Plants Unit, Faculty of Sciences Semlalia, Cadi Ayyad University, CNRST association URAC 35, Marrakech, Morocco;2. Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Science Semlalia, Cadi Ayyad University, Marrakech, Morocco;3. Laboratory of Chemistry, Polydisciplinary Faculty, University Hassan 1er, Khouribga, Morocco;4. Laboratory of Coordination Chemistry and Catalytic, Faculty of Science Semlalia, Cadi Ayyad University, Marrakech, Morocco;1. Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria Technopole, BP 901, 2050 Hammam-Lif, Tunisia;2. Laboratory of Parasitology-Mycology, Pasteur Institute Tunis, LR 11-IPT-06, 13 Place Pasteur, 1002 Tunis, Tunisia;1. Institute of Pharmacology and Toxicology, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria;2. Department of Chemistry, University of Natural Resources and Life Sciences, Tulln, Austria;3. Parasitology Department, Institute of Tropical Medicine “Pedro Kouri”, Havana, Cuba;4. Department of Environmental Geosciences, University of Vienna, Vienna, Austria;1. Departamento de Agronomia, Universidade Federal de Sergipe, Av. Marechal Rondon, S/N, São Cristóvão, Sergipe CEP 49100-000, Brazil;2. Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costabile Romano, 2201 Ribeirania, Ribeirão Preto CEP 14096-000, Brazil;3. Departamento de Química, Universidade Federal de Sergipe, Av. Marechal Rondon, S/N, São Cristóvão, Sergipe CEP 49100-000, Brazil;4. Departamento de Fisiologia, Universidade Federal de Sergipe, Av. Marechal Rondon, S/N, São Cristóvão, Sergipe CEP 49100-000, Brazil;5. Departamento de Morfologia, Universidade Federal de Sergipe, Av. Marechal Rondon, S/N, São Cristóvão, Sergipe CEP 49100-000, Brazil;1. Department of Parasitology, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract:Chenopodium ambrosioides have been used during centuries by native people to treat parasitic diseases.Aims of the studyTo compare the in vivo anti-leishmanial activity of the essential oil (EO) from C. ambrosioides and its major components (ascaridole, carvacrol and caryophyllene oxide).Materials and methodsAnti-leishmanial effect was evaluated in BALB/c mice infected with Leishmania amazonensis and treated with the EO, main compounds and artificial mix of pure components by intralesional route at 30 mg/kg every 4 days during 14 days. Diseases progression and parasite burden in infected tissues were determined.ResultsEO prevented lesion development compared (p < 0.05) with untreated animals and treated with vehicle. In addition, the efficacy of EO was also statistically superior (p < 0.05) compared with the glucantime-treated animals. No potential effects were observed with pure components treatment. Mix of pure compounds cause death of animals after 3 days of treatment.ConclusionsOur results demonstrate the superiority of EO against experimental cutaneous leishmaniasis caused by L. amazonensis.
Keywords:Cutaneous leishmanasis  Ascaridole  Carvacrol  Caryophyllene oxide  Asc"}  {"#name":"keyword"  "$":{"id":"kw0035"}  "$$":[{"#name":"text"  "_":"ascaridole  Car"}  {"#name":"keyword"  "$":{"id":"kw0045"}  "$$":[{"#name":"text"  "_":"carvacrol  Caryo"}  {"#name":"keyword"  "$":{"id":"kw0055"}  "$$":[{"#name":"text"  "_":"caryophyllene oxide  DMSO"}  {"#name":"keyword"  "$":{"id":"kw0065"}  "$$":[{"#name":"text"  "_":"dimethyl sulfoxide  EO"}  {"#name":"keyword"  "$":{"id":"kw0075"}  "$$":[{"#name":"text"  "_":"essential oil  GTM"}  {"#name":"keyword"  "$":{"id":"kw0085"}  "$$":[{"#name":"text"  "_":"glucantime  HRGC-MS"}  {"#name":"keyword"  "$":{"id":"kw0095"}  "$$":[{"#name":"text"  "_":"high resolution gas chromatography-mass spectrometry  p  i  "}  {"#name":"keyword"  "$":{"id":"kw0105"}  "$$":[{"#name":"text"  "_":"post-infection
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