Neuroprotective effect of yokukansan against cytotoxicity induced by corticosterone on mouse hippocampal neurons |
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| Institution: | 1. Department of Pharmacology, School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi 324-8501, Japan;2. Department of Pharmacotherapeutics, School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi 324-8501, Japan;3. Advanced Education and Research Center for Kampo Medicine, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi 324-8501, Japan;1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China;2. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;3. Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China;4. New Technology Center of Chemical Pharmaceutical Shanghai Institute of Pharmaceutical Industry China State Institute of Pharmaceutical Industry, Shanghai 200437, China;5. Jiangxi Qingfeng Pharmaceutical Corporation, Ganzhou 341000, China;1. Department of Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China;2. Department of Naval Hygiene, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China;3. Department of Neurology, No. 411Hospital, 15 East Dongjiangwan Road, Shanghai 200081, China;1. Department of Neurobiology, Kagawa University Faculty of Medicine, Miki, Japan;2. Department of Medical Education, Kagawa University Faculty of Medicine, Miki, Japan;3. Department of Neurological Surgery, Kagawa University Faculty of Medicine, Miki, Japan;4. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA;1. Laboratory of Pharmacology, College of Korean Medicine, Daejeon University, Daejeon 34520, South Korea;2. Department of Neuropsychiatry, Dunsan Korean Medicine Hospital of Daejeon University, Daejeon 35235, South Korea;3. Laboratory of Molecular Medicine, College of Korean Medicine, Daejeon University, Daejeon 34520, South Korea;4. Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, South Korea;5. College of Pharmacy, Gachon University, Incheon 21999, South Korea;6. Department of Internal Medicine, Daejeon Korean Medicine Hospital of Daejeon University, Daejeon 34623, South Korea |
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| Abstract: | Yokukansan, a traditional Japanese herbal medicine, has been used for the management of neurodegenerative disorders and for the treatment of neurosis, insomnia, and behavioral and psychological symptoms of dementia. Recently, several studies have shown that yokukansan has a neuroprotective effect. The aim of this study was to examine the neuroprotective effect of yokukansan on hippocampal neurons from embryonic mouse brain against the effects of corticosterone, which is considered to be a stress hormone and to be cytotoxic toward neurons. The cell survival rates were measured by the WST-8 assay and LDH assay. Twenty-four hours after treatment with corticosterone, cell numbers were significantly decreased compared with the control or treatment with vehicle in a dose-dependent manner. When cells were treated with 30 μM corticosterone, the decrease in the number of cells was significantly recovered by treatment with yokukansan (100–1000 μg/ml) in a dose-dependent manner. However, yokukansan did not suppress the decrease in cell numbers that was induced by treatment with 100 μM corticosterone. In the LDH assay, treatment with yokukansan at a high concentration (500–1000 μg/ml) suppressed the LDH concentration induced by treatment with both 30 μM and 100 μM corticosterone compared to treatment with corticosterone alone, respectively. These results suggest that yokukansan protects against the cytotoxic effect of a low concentration of corticosterone on hippocampal neurons. |
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| Keywords: | Yokukansan (Japanese herbal medicine) Corticosterone Hippocampal neuron WST-8 assay |
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