Synergistic effect of Myrtus communis L. essential oils and conventional antibiotics against multi-drug resistant Acinetobacter baumannii wound isolates |
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| Institution: | 1. Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21 000 Novi Sad, Vojvodina, Serbia;2. Department of Chemistry, Biochemistry and environmental protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21 000 Novi Sad, Vojvodina, Serbia;3. Health Center “Aleksinac”, Hygienic and Epidemiological Service, Momcila Popovica 114, 18220 Aleksinac, Serbia;1. Department of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, Italy;2. CRA-FRU Fruit Trees Research Center, Via di Fioranello 52, 00134 Rome, Italy;3. R.H. Smith Institute of Plant Sciences in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel;4. Department of Chemistry, Bar Ilan University, Ramat Gan, Israel;1. Université Larbi Ben M’Hidi, Oum El Bouaghi, Algeria;2. Laboratoire de l’Ingénierie des Procédés de l’Environnement, Université Constantine 3, Algeria;3. Aix Marseille Université, CNRS, Centrale Marseille, M2P2 UMR 7340, 13451, Marseille, France;1. Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Science Semlalia, University Cadi Ayyad, Marrakech, Morocco;2. Friedrich Loeffler Institute of Medical Microbiology, University of Medicine Greifswald, Germany;3. Biomolecule and Medicinal Chemistry Unit, Faculty of Science, Semlalia University Cadi Ayyad, Marrakech, Morocco;1. CERENA – Centre for Natural Resources and the Environment, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. Universidade Lusófona Research Centre for Biosciences and Health Technologies, ULHT, Av. Campo Grande, 376, 1749-024 Lisboa, Portugal;3. Escola Superior Náutica Infante D. Henrique, Av. Eng. Boneville Franco, 2770-058 Paço d’Arcos, Portugal;4. Centro de Química Estrutural, Complexo Interdisciplinar, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156 83111, Iran;2. Department of Medicinal Plants, Research Centre for Medicinal Plants & Ethno-Veterinary, Shahrekord Branch, Islamic Azad University, PO Box 166, Shahrekord, Iran;1. Centre for Biological and Chemical Engineering, IBB; DEQ, IST, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. Universidade Lusófona de Humanidade e Tecnologias, Av. Campo Grande, 376, 1749-024 Lisboa, Portugal;3. IBB-University of Algarve, Laboratory of Plant Biotechnology, Campus de Gambelas, 8005-139 Faro, Portugal |
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| Abstract: | Acinetobacter baumannii is a rapidly emerging, highly resistant clinical pathogen with increasing prevalence. In recent years, the limited number of antimicrobial agents available for treatment of infections with multi-drug resistant (MDR) strains reinforced tendency for discovery of novel antimicrobial agents or treatment strategies. The aim of the study was to determine antimicrobial effectiveness of three Myrtus communis L. essential oils, both alone and in combination with conventional antibiotics, against MDR A. baumannii wound isolates. The results obtained highlighted the occurrence of good antibacterial effect of myrtle oils when administered alone. Using checkerboard method, the combinations of subinhibitory concentrations of myrtle essential oils and conventional antibiotics, i.e. polymixin B and ciprofloxacine were examined. The results proved synergism among M. communis L. essential oils and both antibiotics against MDR A. baumannii wound isolates, with a FIC index under or equal 0.50. Combination of subinhibitory concentrations of essential oils and ciprofloxacin most frequently reduced bacterial growth in synergistic manner. The similar has been shown for combination with polymyxin B; furthermore, the myrtle essential oil resulted in re-sensitization of the MDR wound isolates, i.e. MICs used in combination were below the cut off for the sensitivity to the antibiotic. Time-kill curve method confirmed efficacy of myrtle essential oil and polymyxin B combination, with complete reduction of bacterial count after 6 h. The detected synergy offers an opportunity for future development of treatment strategies for potentially lethal wound infections caused by MDR A. baumannii. |
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| Keywords: | Antimicrobial agents Synergism Multi-drug resistant |
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