Hepatic metabolite profiles in mice with a suboptimal selenium status |
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| Affiliation: | 1. ZIEL Research Center of Nutrition and Food Sciences, Department of Biochemistry, Technical University of Munich, Gregor-Mendel-Strasse 2, 85350 Freising, Germany;2. Institute of Experimental Endocrinology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany;3. Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany;1. Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;2. Departments of Medicine and Genetics, Albert Einstein College of Medicine, NY 10461, USA;3. Department of Microbiology and Immunology, Drexel University College of Medicine, PA 19129, USA;4. Department of Pathology, Montefiore Medical Center, NY 10467, USA;1. Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan;2. Division of Histological and Clinical Pathology, Hualian Army Forces General Hospital, Hualien, Taiwan;3. Neural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan;4. Department of Food Technology, Tajen University, Pingtung, Taiwan;1. National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, India 160071;2. National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, India 160062;3. Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India 160025;4. Department of Biochemistry, Panjab University, Chandigarh, India 160014;5. Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL 62702, USA;1. INRAE, Université de Pau et des Pays de l''Adour, E2S UPPA, NUMEA, Saint-Pée-sur-Nivelle, France;2. Université de Pau et des Pays de l''Adour, E2S UPPA, CNRS, IPREM, Pau, France;3. Phileo by Lesaffre, 59700 Marcq-en-Barœul, France |
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| Abstract: | Selenium is an essential trace element and mediates its functions via various selenoproteins such as glutathione peroxidases or thioredoxin reductases. A suboptimal selenium supply causes metabolic disturbances and is associated with an increased risk to develop different disorders, including cancer or cardiovascular diseases. This study aimed to assess the impact of a suboptimal selenium status on the hepatic metabolome of male mice analyzed by a targeted liquid chromatography/tandem mass spectrometry and a method based on non-targeted gas chromatography hyphenated with mass spectrometry. Feeding animals a diet with about half of the recommended selenium content supplied as selenomethionine caused liver glutathione peroxidase and thioredoxin reductase activities to decline and lipid peroxidation to increase. Serum T3 thyroid hormone concentration also declined via a reduced hepatic deiodinase activity. Metabolite profiling revealed predominantly changes in cysteine and carbon-1 metabolism as well as in selected lipid subclasses. In particular the concentrations of palmitoylcarnitines and oleoylcarnitines (C18:1 and C16:1) and various phosphatidylcholine species containing saturated fatty acids were elevated. Increased taurine levels suggested an enhanced cysteine flux through the salvage pathway whereas increased homocysteine levels appeared to be a consequence of a massive down-regulation of cystathionine β lyase (cystathionine β synthase) and a reduced flux through the transsulfuration pathway. The findings demonstrate that a suboptimal selenium status causes alterations in lipid and carbon-1 metabolism in mouse liver. These changes may contribute to the development of diseases associated with a suboptimal selenium status. |
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