Oroxylin A,a constituent of Oroxylum indicum inhibits adipogenesis and induces apoptosis in 3T3-L1 cells |
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| Affiliation: | 1. Department of Physical Therapy, Inje University, Gimhae, 621-749, Republic of Korea;2. Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea;3. Department of Biotechnology, Catholic University of Daegu, Gyeongsan 712-702, Republic of Korea;4. Magae Bioscience Institute, 49-4 Fujimidai, Tsukuba 300-1263, Japan;1. Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, Matsuyama University, 4-2 Bunkyo-cho, Matsuyama, Ehime, 790-8578, Japan;2. Department of Biochemistry, College of Pharmaceutical Sciences, Matsuyama University, 4-2 Bunkyo-cho, Matsuyama, Ehime, 790-8578, Japan;3. Department of Pharmacognosy, College of Pharmaceutical Sciences, Matsuyama University, 4-2 Bunkyo-cho, Matsuyama, Ehime, 790-8578, Japan;4. National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-9501, Japan;1. Department of Biology, Jeju National University, 102 Jejudaehakno, Jejusi, Jeju Special Self-Governing Province 690-756, Republic of Korea;2. Jeju Sasa Industry Development Agency, Jeju National University, 102 Jejudaehakno, Jejusi, Jeju Special Self-Governing Province 690-756, Republic of Korea;1. Herbal Research Section, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, India;2. Central Pathology Lab, CSIR-Indian Institute of Toxicology Research, Lucknow 226001, India;1. Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210023, China;2. Department of Pathogenic biology and Immunology, Medical School, Southeast University, Nanjing, 210009, China;3. Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210023, China;4. Department of Pathology, School of Medicine, Saint Louis University, St Louis, MO, 63104, USA;5. Shandong co-innovation center of TCM formula, College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, China |
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| Abstract: | Oroxylin A (OA) is a flavonoid found in Oroxylum indicum, a medicinal plant with multiple biological activities. This study was taken up to investigate the effect of OA, on adipogenesis, lipolysis and apoptosis in 3T3 L1 cells. Pre-adipocytes were treated with 10–40 μM OA on various days of adipogenesis treatment schedule. Mature adipocytes were treated with OA for lipolysis and apoptosis studies. In maturing pre-adipocytes, 10 μM OA suppressed intracellular lipid accumulation by 42.19% which was confirmed by lipidTox imaging of cells. In addition, OA decreased the nuclear translocation of PPARγ and mRNA expression of its downstream genes (FAS and LPL) along with adiponectin secretion. In mature adipocytes, 40 μM of OA decreased cell viability by 30% of control. Annexin V/PI staining showed induction of apoptosis which was further confirmed by enhanced levels of pro-apoptotic proteins Bax, cyt c, AIF and chromatin condensation. OA enhanced TNF-α secretion, lipolysis and decreased Akt phosphorylation in mature adipocytes. Findings suggest that OA possibly exerts its anti-obesity effect by affecting adipocyte life cycle at critical points of differentiation and maturity. When we compared the potency of OA with non-methoxylated flavonoids morin, naringenin and kaempferol on adipocyte life cycle OA was far more potent. Thus, study clearly indicates a new role for oroxylin A as regulator of adipocyte life cycle. In addition, study also suggested a specific role of methoxylated group in exerting lipolysis and cytotoxic effects in mature adipocytes. |
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| Keywords: | Oroxylin A Adipogenesis Lipolysis Apoptosis PPARγ |
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