Statistical accuracy in fluorescence fluctuation experiments |
| |
Authors: | Peet Kask R Günther Peter Axhausen |
| |
Institution: | (1) EVOTEC BioSystems GmbH, Grandweg 64, D-22529 Hamburg, Germany (Fax +49-40-56081222; e-mail: guentherevotec.de), DE |
| |
Abstract: | The mathematical expression of the signal to noise ratio in fluorescence fluctuation experiments is derived for arbitrary
sample profiles and for any mechanism of translational motion, and experimentally verified. The signal to noise ratio depends
on the mean count rate per particle per dwell time, the mean number of particles per sample volume, time characteristics of
the correlation function, sample profile characteristics, and the data collection time. Statistical accuracy of the third
order moment of fluorescence intensity fluctuations is also studied. The optimum concentration for the third order moment
analysis is about one particle per sample volume.
Received: 13 February 1996 / Accepted: 20 September 1996 |
| |
Keywords: | Fluorescence correlation spectroscopy Fluorescence intensity fluctuations Fluorescence intensity distribution analysis |
本文献已被 SpringerLink 等数据库收录! |
|