Cyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of Paenibacillus graminis |
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| Authors: | Renata Estebanez Vollú Fabio Faria da Mota Eliane Aparecida Gomes Lucy Seldin |
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| Affiliation: | 1.Laboratório de Genética Microbiana,Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brasil;2.EMBRAPA/CNPMS,Sete Lagoas,Brazil |
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| Abstract: | Paenibacillus graminis strains were described recently as cyclodextrin (CD) producers. Cyclodextrins are produced by cyclodextrin glucanotransferase (CGTase) which has not been characterized in P. graminis. Similar amounts of α- and β-CDs were produced by P. graminis (MC22.13) and P. macerans (LMD24.10T). Primers were designed to sequence the gene encoding CGTase from P. graminis. A phylogenetic tree was constructed and P. graminis CGTase protein showed to be closer (79.4% protein identity) to P. macerans |P31835|. Hybridization studies suggested that the gene encoding CGTase is located in different positions in the genomes of P. macerans and P. graminis. |
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| Keywords: | Cyclodextrin Cyclodextrin glucanotransferase Nucleotide sequence Paenibacillus graminis Phylogeny |
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