Paternity testing of wild black rockfish <Emphasis Type="Italic">Sebastes inermis</Emphasis> (brownish type) from the Seto Inland Sea of Japan |
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| Authors: | Enrique Blanco Gonzalez Tomoya Murakami Yuki Teshima Kouji Yoshioka Dal-Sang Jeong Tetsuya Umino |
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| Institution: | (1) Graduate School of Biosphere Sciences, Hiroshima University, Higashi-Hiroshima Hiroshima, 739-8528, Japan;(2) Hiroshima Prefectural Technology Research Institute Fisheries and Ocean Technology Center, Kure Hiroshima, 737-1207, Japan;(3) Hiroshima Prefectural Fisheries Coordination Office, Hiroshima Hiroshima, 730-8511, Japan;(4) Marine Seed Releasing Technology Center, 591 Jeongdori, Wando, 537-806, South Korea |
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| Abstract: | We developed four microsatellite DNA loci to test for multiple paternity of black rockfish, Sebastes inermis, from the Seto Inland Sea of Japan. All loci showed a high degree of polymorphism (number of alleles per locus = 10–14, expected
heterozygosity = 0.80) and discriminating power (probability of identity index = 3.71 × 10−6, exclusion probability = 0.999) in unrelated wild specimens (n = 32). Genotypic assignment of five dams (109–220 mm in total length) and 50 embryos from each dam (n = 50) indicated that four dams were mated with a single sire. Only for one dam and three of her embryos we could not exclude
multiple paternity. |
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| Keywords: | Black rockfish Sebastes inermis Microsatellite DNA Mating Paternity diagnosis |
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