Apoptosis detected in hybrids between Nicotiana glutinosa and N. repanda expressing lethality |
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Authors: | Wataru Marubashi Tetsuya Yamada Masaru Niwa |
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Institution: | (1) Laboratory of Plant Breeding, School of Agriculture, Ibaraki University, 3-21-1 Chuo, Ami-machi, Ibaraki-ken, 300-0393, Japan, JP |
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Abstract: | Hybrid lethality is one of the mechanisms for reproductive isolation. Apoptotic features were detected in the cells of hybrid
seedlings of Nicotiana glutinosa L. ×N. repanda Willd. Condensation of chromatin and fragmentation of nuclei were observed in the leaf protoplasts isolated from hybrid seedlings
expressing this lethality. Fragmentation of nuclei was correlated with the progression of lethal symptoms, as confirmed by
fluorimetry of the nuclear DNA using laser scanning cytometry. Agarose gel analysis of DNA extracted from hybrid leaves showing
lethality revealed a specific ladder pattern suggesting nucleosomal fragmentation associated with nuclear fragmentation. In-situ
detection of DNA fragmentation using terminal deoxyribonucleotidyl transferase-mediated dUTP-fluorescein nick end labeling
(TUNEL) showed that this process occurred in all leaf cells. This is the first evidence that apoptosis can induce suicide
of hybrid plants, thus leading to reproductive isolation.
Received: 18 June 1999 / Accepted: 20 July 1999 |
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Keywords: | Key words: Apoptosis Chromatin condensation DNA fragmentation Hybrid lethality Interspecific hybrid Nicotiana(apoptosis) |
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