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Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157
Authors:Huijuan Liu  Zaibao Zhang  Hui Li  Jufang Gao  Zhongnan Yang
Affiliation:1. College of Life and Environment Sciences, Shanghai Teachers University, Shanghai, 200234, China
Abstract:An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy.Genetic analysis indicated that it was controlled by a single recessive gene called ms157.No pollen grains have been observed in mutant anthers.ms157 Has been mapped to a region of 74 kb located in BAC clone T6K22 on chromosome Ⅳ using a map-based cloning strategy.As no male sterile genes have been reported in this region.ms157 could be a novel gene related to fertility.The further molecular cloning and functional analysis on this gene should facilitate our understanding of A.thaliana anther development.
Keywords:EC2-157  anther development  male sterile  map-based cloning  Arabidopsis thaliana
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