Extraction,purification and characterization of an antioxidant from marine waste using protease and chitinase cocktail |
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Authors: | N N Nawani D Prakash B P Kapadnis |
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Institution: | (1) Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Akurdi, Pune, 411044, India;(2) Department of Microbiology, University of Pune, Pune, Maharashtra, 411007, India |
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Abstract: | Marine waste is a highly renewable resource for the recovery of several value added metabolites with prospective industrial
applications. This study describes the production of enzymes on marine waste and their subsequent use for the extraction of
antioxidants from marine waste. Microbispora sp. and Bacillus sp. were grown on colloidal chitin and marine waste for the production of chitinase and protease. Microbispora sp. could produce 10.2 U ml−1 chitinase, whereas Bacillus sp. could produce 38 U ml−1 chitinase and 3.39 U ml−1 protease. The production of antioxidants was optimized using statistical designs and 6.6 units of 35 kDa chitinase from Microbispora sp., 16 units of 25 kDa chitinase from Bacillus sp., 2.3 units of protease, 1.5% marine waste and 36 h incubation gave maximum antioxidant activity. Nearly 5.0 mg of compound
with antioxidant activity could be recovered per gram of marine waste. This compound was purified by HPLC and characterized
by TLC, FT-IR and proton-NMR as N,N′-diacetylchitobiose. It exhibited 53% superoxide radical scavenging activity, 57% hydroxyl radical scavenging activity and
28% lipid peroxidation inhibition activity. Scale up of the extraction of antioxidant from marine waste and its pharmacological
studies can extend its use in medicine. |
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