Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500 |
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| Authors: | Mingqiu Liu Xiaofeng Niu Jingran Yan Weiyao Yan Zhaoxin Zheng |
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| Affiliation: | 1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, China
2. Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, Shanghai, 200031, China
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| Abstract: | A recombinant strain of Salmonella choleraesuis C500,containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus,was constructed.Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S.cho in rabbits.Results showed that T cell response and specific antibody production were elicited.This approach may present a general strategy for eliciting immune responses with DNA vaccine delivered by live bacterial vectors.The stimulated indexes of T lymphoproliferation by specific antigens of FMDV in rabbits,can reach up to 11.0 and an antibody titer of 1/32 as detected in the erum with liquid block ELISA. |
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| Keywords: | Salmonella choleraesuis foot-and-mouth disease virus recombinant live vaccine |
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