Bioconversion of butyronitrile to butyramide using whole cells of <Emphasis Type="Italic">Rhodococcus rhodochrous</Emphasis> PA-34 |
| |
Authors: | J Raj A Seth S Prasad T C Bhalla |
| |
Institution: | (1) Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171 005, India |
| |
Abstract: | Butyramide is an important chemical commodity, which is used for the synthesis of hydroxamic acids and electrorheological
fluids and for the preparation of β-amodoorganotin compounds. The nitrile hydratase (Nhase) of Rhodococcus rhodochrous PA-34 catalyzed the conversion of butyronitrile to butyramide. The maximum Nhase activity 18 U/mg dry cell weight (dcw)]
of whole cells of R. rhodochrous PA-34 was observed at pH 7.0 with 10% (v/v) butyronitrile and 1 mg cells (dcw)/ml reaction mixture at 10°C. The cells of
R. rhodochrous PA-34 retained almost 50% activity when incubated for 1 h in the presence of 85% (v/v) butyronitrile. A yield of 597 g of
butyramide (6.8 M) was obtained using 60% (v/v) butyronitrile, 1 g cells (dry weight) in a 1-l batch reaction at 10°C for
6 h. |
| |
Keywords: | Rhodococcus rhodochrous PA-34 Nitrile hydratase Butyronitrile Butyramide |
本文献已被 PubMed SpringerLink 等数据库收录! |