Effects of light conditions and 2,4-D concentration in soybean anther culture |
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Authors: | Email author" target="_blank">LR?RodriguesEmail author B de C?Forte JMS?Oliveira JEA?Mariath MH?Bodanese-Zanettini |
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Institution: | (1) Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box 15053, CEP 91501-970 Porto Alegre, RS, Brazil;(2) Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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Abstract: | The morphogenic response of anther walls and connective tissue is the greatest obstacle to androgenesis in soybean anther culture. Whereas induction to microspore embryogenesis occurs in the dark in almost all plant species, soybean anthers have been cultured under light. In an attempt to establish culture conditions that simultaneously stimulate microspore embryogenesis and inhibit epidermal and connective cell proliferation, the effect of light and two 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations (2 and 10 mg l–1) on the induction process was investigated. Higher 2,4-D concentration speeded up microspore plasmolysis and did not improve androgenesis. Callogenesis and embryogenesis induction from sporophytic cells were significantly lower in the dark, and some microspores showed major alterations in the sporoderm. Auxin 2,4-D and induction under light contributed to the morphogenic response of the anther walls and connective tissue under the conditions previously recommended to trigger microspore embryogenesis. |
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Keywords: | Androgenesis Culture conditions 2 4-D Glycine max Light Somatic embryogenesis |
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