Nerve Growth Factor Protects the Cortical Neurons from Chemical Hypoxia-induced Injury |
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| Authors: | Li Zhu Fang Du Lei Yang Xiao Mei Wu Zhong Ming Qian |
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| Institution: | (1) Institute for Nautical Medicine and Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, P.R. China;(2) Laboratory of Iron Metabolism, Department of Applied Biology & Chemical Technology and National Key Laboratory of Chinese Medicine and Molecular Pharmacology (Shenzhen), Hong Kong Polytechnic University, Kowloon, Hong Kong |
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| Abstract: | Previous studies showed that nerve growth factor (NGF) exerts protective effects on cultured neurons against various kinds
of damage. However, a recent publication reported that exposure of NGF-treated PC12 cells to physical hypoxia resulted in
a higher cell death rate when compared to the untreated controls. In the present study, we therefore investigated the effects
of NGF on the hypoxic cortical neurons induced by potassium cyanide (KCN). We demonstrated that NGF at a higher concentration
can significantly increase neuronal viability, decrease the release of lactate dehydrogenase and improve cellular morphology
in the hypoxic cortical neurons. However, we also found that pretreatment of NGF was not able to completely revise the decreased
cell viability and the increased leakage of lactate dehydrogenase (LDH) induced by KCN, although the indexes in the neurons
treated with NGF and KCN were significantly higher than those in the neurons treated with KCN only. Analysis of the data showed
that the incomplete revision of NGF should be not due to the dosage of NGF we used. It might be induced by the inability of
NGF to inhibit all injury pathways induced by potassium cyanide. |
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| Keywords: | Nerve growth factor (NGF) Neuroprotection Primary cortical neurons Neuronal viability Lactate dehydrogenase (LDH) Cellular morphology Potassium cyanide (KCN) |
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