Activation of chicken liver fructose-1,6-bisphosphatase by oxidized glutathione |
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Authors: | D Neuser P Bellemann |
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Affiliation: | 1. Dolpus E. Milligan Science Research Institute, Atlanta University Center, Atlanta, GAUSA 30310;2. Morehouse School of Medicine, Atlanta, GA 30310, USA;3. Morehouse College, Atlanta, GA 30310, USA |
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Abstract: | Treatment of chicken liver fructose-1,6-bisphosphatase with oxidized glutathione (GSSG) leads to an increase in activity. This activation is markedly enhanced if treatment is performed in the presence of AMP or Mn2+. The effects of AMP and Mn2+ appear to be synergistic. The maximal activation is over 13-fold and is accompanied by the disappearance of 4 sulfhydryl groups per molecule of enzyme. Both fructose 1,6-bisphosphate and fructose 2,6-bisphosphate can largely prevent this activation. Activation can be reversed by dithiothreitol or cysteine. It appears that GSSG activates this enzyme by thiol/disulfide exchanges with the enzyme's specific sulfhydryl groups. |
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Keywords: | Glutathione Enzyme activation Fructose-1,6-bisphosphatase (Chicken liver) |
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