Residual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species |
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Authors: | M-K Kim I-Y Lee J-H Lee K-T Kim Y-H Rhee Y-H Park |
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Institution: | (1) Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, South Korea, KR;(2) Department of Chemical Engineering, Chosun University, 501 Kwangju, South Korea,;(3) Department of Microbiology, Chungnam National University, 220 Kungdong, Taejon 305-764, South Korea , |
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Abstract: | We investigated the influence of inorganic phosphate concentration on the production of curdlan by Agrobacterium species. A two-step culture method was employed where cells were first cultured, followed by curdlan production under nitrogen-limiting
conditions. In the curdlan production step, cells did not grow but metabolized sugar into curdlan. Shake-flask experiments
showed that the optimal phosphate concentration for curdlan production was in the range of 0.1–0.3 g l−1. As the cell concentration increased from 0.42 to 1.68 g l−1 in shake-flask cultures, curdlan production increased from 0.44 to 2.80 g l−1. However, the optimal phosphate concentration range was not dependent upon cell concentration. The specific production rate
was about 70 mg curdlan g-cell−1 h−1 irrespective of cell concentration. When the phosphate concentration was maintained at 0.5 g l−1 under nitrogen-limiting conditions, as high as 65 g l−1 of curdlan was obtained in 120 h. Journal of Industrial Microbiology & Biotechnology (2000) 25, 180–183.
Received 25 October 1999/ Accepted in revised form 21 July 2000 |
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Keywords: | : curdlan production phosphate concentration Agrobacterium sp |
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