A cytokinetic model for heterogeneous mammalian cell populations. III. Tritiated thymidine studies: Correlations among multiple kinetic parameters in human tumors |
| |
Authors: | Stanley E. Shackney |
| |
Affiliation: | Senior Investigator, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014, U.S.A. |
| |
Abstract: | A computer model for the simulation of radioautographic studies in growing mammalian cell populations was used to study multiple tritiated thymidine (3HTdR) associated parameters in model populations whose properties are comparable to those found in human tumors. Several different DNA synthesis rate patterns are distinguishable, designated type I, intermediate, and type II. Correlations among percent labelled mitosis (PLM) curves, interphase cell labelling patterns, and continuous 3HTdR labelling studies suggest a type I pattern in human breast cancer, an intermediate pattern in human melanoma, and a type II pattern in human adult leukemia. Detailed simulation studies were carried out in human adult acute leukemia and human melanoma. It was possible to fit all available kinetic data in leukemia and melanoma, both with respect to low threshold data, and with respect to radioautographic labelling intensity, provided that simulated experimental conditions and simulated radioautographic conditions corresponded to those actually employed. Kinetic differences between leukemia and melanoma were demonstrated which are in keeping with the natural histories and clinical drug response behavior of these two malignancies. |
| |
Keywords: | Reprint requests should be addressed to Dr. Stanley E. Shackney National Institutes of Health 9000 Rockville Pike Bethesda Maryland 20014. |
本文献已被 ScienceDirect 等数据库收录! |
|