Rapid determination of isoamyl nitrite in pharmaceutical preparations by flow injection analysis with on‐line UV irradiation and luminol chemiluminescence detection |
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| Authors: | Naoya Kishikawa Naoko Kondo Abena Amponsaa‐Karikari Hitoshi Kodamatani Kaname Ohyama Kenichiro Nakashima Shigeo Yamazaki Naotaka Kuroda |
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| Affiliation: | 1. Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan;2. Faculty of Science, Kagoshima University, Kagoshima, Japan;3. Department of Applied Science, Okayama University of Science, Okayama, Japan |
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| Abstract: | Isoamyl nitrite is used as a therapeutic reagent for cardiac angina and as an antidote for cyanide poisoning, but it is abused because of its euphoric properties. Therefore, a method to determine isoamyl nitrite is required in many fields, including pharmaceutical and forensic studies. In this study, a simple, rapid and sensitive method for the determination of isoamyl nitrite was developed using a flow injection analysis system equipped with a chemiluminescence detector and on‐line photoreactor. This method is based on on‐line ultraviolet irradiation of isoamyl nitrite and subsequent luminol chemiluminescence detection without the addition of an oxidant. A linear standard curve was obtained up to 1.0 μM of isoamyl nitrite with a detection limit (blank + 3SD) of 0.03 μM. The method was successfully applied to determine isoamyl nitrite content in pharmaceutical preparations. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | alkyl nitrite luminol chemiluminescence photochemical reaction flow injection analysis |
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