Electrophoresis–chemiluminescence detection of phenols catalyzed by hemin |
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Authors: | Lu Shu Jinkun Zhu Qingjiang Wang Pingang He Yuzhi Fang |
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Institution: | 1. Department of Chemistry, East China Normal University, Shanghai, People's Republic of China;2. College of Science, Anhui Science and Technology University, Fengyang, People's Republic of China |
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Abstract: | Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis–chemiluminescence (CE‐CL) detection method for phenols using a hemin–luminol–hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br? and F? could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE‐CL detection system because of the self‐polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin–luminol afforded a stable CE‐CL baseline. The indirect CE‐CL detection of five phenols using this method gave the following limits of detections: 4.8 × 10?8 mol/L (o‐sec‐butylphenol), 4.9 × 10?8 mol/L (o‐cresol), 5.4 × 10?8 mol/L (m‐cresol), 5.3 × 10?8 mol/L (2,4‐dichlorophenol) and 7.1 × 10?8 mol/L (phenol). Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | capillary electrophoresis chemiluminescence hemin phenol detection |
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