Development and characterization of a novel bioactive polymer with antibacterial and lysozyme‐like activity |
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| Authors: | Maria Rachele Guascito Daniela Chirizzi Cosimino Malitesta Livia Giotta Disma Mastrogiacomo Ludovico Valli Loredana Stabili |
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| Institution: | 1. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy;2. Dipartimento di Beni Culturali, Università del Salento, Lecce, Italy;3. Istituto per l'Ambiente Marino Costiero CNR U.O.S. Taranto, Taranto, Italy |
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| Abstract: | The development and characterization of a novel bioactive polymer based on the immobilization of glucose oxidase enzyme (GOx) in a polyvinyl alcohol (PVA) film showing antibacterial activity is presented. The PVA‐GOx composite material was extensively characterized by UV‐vis, X‐ray Photoelectron (XPS) spectroscopy and by Fourier Transform Infrared (FTIR) spectroscopy to verify the preservation of enzyme structural integrity and activity. The antimicrobial activity of this composite material against Escherichia coli and Vibrio alginolyticus was assessed. Furthermore the lysozyme‐like activity of PVA‐GOx was highlighted by a standard assay on Petri dishes employing Micrococcus lysodeikticus cell walls. The findings from this study have implications for future investigations related to the employment of PVA‐GOx system as a composite material of pharmaceutical and technological interest. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 461–470, 2014. |
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| Keywords: | antibacterial glucose oxidase lysozyme‐like activity polyvinyl alcohol XPS FTIR |
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