In vitro production of sedative galphimine B by cell suspension cultures of Galphimia glauca |
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| Authors: | Lidia Osuna Rogelio Pereda-Miranda M Luisa Villarreal |
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| Institution: | (1) Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, 62210, México;(2) Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Xochitepec, Morelos, 62790, México;(3) Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, D.F. 04510, México |
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| Abstract: | The sedative triterpene, galphimine B (1), was detected in cell suspension-batch cultures of Galphimia glauca. The effect of inoculum size, growth regulators and different concentrations of sucrose, nitrates and phosphates was evaluated. A two-stage batch process for biomass production and accumulation of compound 1 was established. Major cellular growth (15 g l–1 dry wt) was obtained in the first stage with naphthaleneacetic acid (2 mg l–1) + kinetin (2 mg l–1). Adding 4 mg 2,4-dichlorophenoxyacetic l–1 acid in the second stage resulted in the highest accumulation of 1 (0.21 mg g–1 dry wt) which was 36% higher with respect to calluses and comparable to that obtained from wild plants. |
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| Keywords: | 6-acetoxygalphimine B cell suspension cultures galphimine B Galphimia glauca two-stage culture |
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