MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1 |
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Authors: | Zhen Liang Shiqi Li Xin Xu Xianglai Xu Xiao Wang Jian Wu Yi Zhu Zhenghui Hu Yiwei Lin Yeqing Mao Hong Chen Jindan Luo Ben Liu Xiangyi Zheng Liping Xie |
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Affiliation: | Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China |
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Abstract: | MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3′-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3′-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1. |
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Keywords: | bladder cancer cyclin D1 microRNA-576-3p proliferation |
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