豌豆茎基腐病病原菌分离鉴定及其对杀菌剂的敏感性测定

豌豆(Pisum sativum)是我国重要的豆类经济作物, 病害对豌豆生产造成重大经济损失。通过形态学观察、分子鉴定以及致病性测定, 最终确定引起豌豆茎基腐病的3种病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、芸苔链格孢菌(Alternaria brassicae)和格氏镰刀菌(F. grosmichelii), 优势菌株为尖孢镰刀菌, 分离率为53.6%。室内毒力测定结果表明, 5种供试杀菌剂对3种病原菌的菌丝生长均有抑制作用, 其中咯菌腈和戊唑醇的抑菌效果最好。研究结果为豌豆茎基腐病的防治提供了科学依据。

Pathogen Identification of Pea Crown Rot and Its Sensitivity to Fungicides

Pea (Pisum sativum) is one of the most important economic crops of beans in China. The occurrence of diseases will cause great economic losses in pea production. Through morphological observation, molecular identification and pathogenicity determination, the results showed that the three kinds of pathogenic fungi causing pea crown rot were Fusarium oxysporum, Alternaria brassicae and F. grosmichelii, among which F. oxysporum was the most pathogenic, with the isolation frequency of 53.6%. The results of indoor toxicity measurement showed that five fungicides had inhibitory effects on the mycelial growth of three kinds of pathogenic bacteria, among which, the inhibition effect of fludioxonil and tebuconazole have top results. The results provide a scientific basis for the prevention and control of pea crown rot.