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Nuclear localization of yeast Nfs1p is required for cell survival
Authors:Nakai Y  Nakai M  Hayashi H  Kagamiyama H
Institution:Department of Biochemistry, Osaka Medical College, Takatsuki, Osaka 569-8686 Japan. med004@art.osaka-med.ac.jp
Abstract:Saccharomyces cerevisiae Nfs1p is mainly found in the mitochondrial matrix and has been shown to participate in iron-sulfur cluster assembly. We show here that Nfs1p contains a potential nuclear localization signal, RRRPR, in its mature part. When this sequence was mutated to RRGSR, the mutant protein could not restore cell growth under chromosomal NFS1-depleted conditions. However, this mutation did not affect the function of Nfs1p in biogenesis of mitochondrial iron-sulfur proteins. The growth defect of the mutant was complemented by simultaneous expression of the mature Nfs1p, which contains the intact nuclear localization signal but lacks its mitochondrial-targeting presequence. These results suggest that a fraction of Nfs1p is localized in the nucleus and is essential for cell viability.
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