Changes in chloroplast number during pea leaf development |
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Authors: | Gayle K Lamppa Leslie V Elliot Arnold J Bendich |
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Institution: | (1) Department of Botany, AJ-10, University of Washington, 98195 Seattle, WA, USA |
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Abstract: | Protoplasts were prepared from pea (Pisum sativum L.) leaves throughout development and their contents spread in a monolayer to determine the number of chloroplasts per cell.
This approach permitted the rapid analysis of more than 100 cells at each stage of development. The average number of chloroplasts
per cell increased from 24±10 to 64±20 during greening and expansion of the first true foliage leaves; all cells containing
chloroplasts apparently increase their chloroplast number. A parallel increase in the amount of DNA per nucleus was not observed.
As the leaves senesced the chloroplast number gradually decreased to 44±12. We have correlated these changes with our previous
results on the percentage of chloroplast DNA per cell. Chloroplast multiplication resulted in a 2.7-fold dilution (from 272
to 102) of the number of copies of the chloroplast DNA molecule per plastid. |
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Keywords: | Chloroplasts (number DNA) DNA (chloroplast) Pisum Protoplasts |
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