TDZ高效诱导蝴蝶兰叶片不定芽及植株再生

以蝴蝶兰（Phalaenopsis）无菌幼苗叶片为材料,研究添加TDZ（噻重氮苯基脲）条件下不同基因型、激素组合、叶片大小、暗培养时间对不定芽发生和再生植株的影响。结果表明：在相同培养条件下,不同基因型外植体芽诱导率差异显著,‘红天使’最高,达81.5%,‘汕农姑娘’等2个品种为0,‘满天红’等4个品种为9.2%～34.9%;添加TDZ芽诱导率显著高于6-BA;单独添加TDZ或6-BA芽诱导率显著高于NAA与TDZ或6-BA的组合。叶片越小不定芽诱导率越高;短时间暗处理有利于不定芽的发生。以1～2 cm长叶片为材料、15 d暗处理、在1/2 MS添加3 mg/L TDZ培养基中,‘红天使’的叶片外植体芽诱导率和平均不定芽数分别可达100.0%和18.2个。研究发现,在继代培养中TDZ对芽的伸长有抑制作用。

The High-frequency Induction of Adventitious Shoots and Plant Regeneration from Leaf Explants of Phalaenopsis with Added Thidiazuron

The effects of different genotypes,plant growth regulators(PGRs) combination,leaf size and dark treatment on adventitious shoot formation and plant regeneration from leaf explants of Phalaenopsis with thidiazuron(TDZ) added were studied.The difference in the induction rate of adventitious shoots among different genotypes with same induction conditions was significant,for example,the 'Red Angle' was the highest,up to 81.5%,Doritaenopsis Shannong 'Lassie' and one other were 0,Doritaenopsis Queen Beer 'Red Sky...