Inducing homozygosity in transgenic barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) by microspore culture |
| |
Authors: | Email author" target="_blank">Anneli?RitalaEmail author Salla?Marttila Annika?Wilhelmson Anna?Maria?Nuutila |
| |
Institution: | (1) VTT Biotechnology, P.O.Box 1000, FI-02044 VTT, Finland;(2) Department of Crop Science, Swedish University of Agricultural Sciences, P.O.Box 44, SE-23053 Alnarp, Sweden |
| |
Abstract: | Homozygosity was induced in transgenic barley by microspore culture. Spikes of transgenic barley plants carrying microspores
in the late uni-nucleate stage were cold pretreated. Teflon rod maceration and a density of 100 000 viable micropores per
plate were used. The developed calli were regenerated and plantlets were treated with colchicine. The microspore culture of
16 mother plants (three transgenic lines) resulted in 927 green regenerants. Of these plants, 476 were transferred to soil,
380 were transgenic, 358 reached maturity and 350 were fertile with a normal seed-set carrying a yield of 6.9 kg. A production
efficiency of 0.8 fertile transgenic doubled haploid barley plants per spike used for microspore isolation was recorded. The
produced transgenic seeds were used in malting experiments. |
| |
Keywords: | barley doubled haploid endo-β -glucanase microspore culture transgenic |
本文献已被 SpringerLink 等数据库收录! |
|