Regulation of cyclic GMP, cyclic AMP and lactate dehydrogenase by putative neurotransmitters in the C6 rat glioma cell line. |
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Authors: | J E Bottenstein J de Vellis |
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Affiliation: | Departments of Anatomy and Neuroscience, and the Laboratory of Nuclear Medicine and Radiation Biology, UCLA School of Medicine, University of California Los Angeles, California 90024, USA |
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Abstract: | In C6 cells norepinephrine and dopamine caused transient increases in cyclic GMP and cyclic AMP, as well as an induction of lactate dehydrogenase. All of these responses were blocked by l-propranolol, suggesting mediation by a β-receptor. Phentolamine potentiated the NE-increased cAMP levels by 5-fold when NE was used at suboptimal doses, suggesting the presence of α-adrenergic receptors in C6 cells. Carbamylcholine decreased the levels of both cyclic nucleotides, with hexamethonium partially reversing the effect on cyclic GMP. Dibutyryl-cyclic GMP or carbamylcholine reduced catecholamine-induced cyclic AMP levels. Serotonin increased cyclic GMP levels 60% and decreased cyclic AMP levels 36%. Calcium- and magnesium-free media inhibited the norepinephrine-induced levels of cyclic GMP and cyclic AMP respectively. |
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Keywords: | To whom reprint requests should be addressed at Warren Hall 900 Veteran Ave. Los Angeles California 90024. |
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