Metaphase arrest and cell death induced by etoposide on HeLa cells |
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Authors: | Rello-Varona Santiago Gámez Angelo Moreno Vanessa Stockert Juan Carlos Cristóbal Javier Pacheco María Cañete Magdalena Juarranz Angeles Villanueva Angeles |
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Affiliation: | aDepartamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, C/ Darwin 2, Citología A-115, E-28049 Madrid, Spain |
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Abstract: | DNA damage, cell cycle and apoptosis form a network with important implications for cancer chemotherapy. Dysfunctions of the cycle checkpoints can allow cancer cells to acquire drug resistance. Etoposide is a well-known inducer of apoptosis, which is widely used in cell biology and in clinical practice. In this work we report that a pulse of 50 μM etoposide (incubation for only 3 h) on HeLa cells causes a sequence of events that leads to abnormal mitotic figures that could be followed either by cell death or, more commonly, by interphase restitution and endocycle. The endocycling polyploid cells enter immediately into mitosis and suffer metaphase blockage with multiple spindle poles, which were generally followed by a direct triggering of apoptosis from metaphase (mitotic catastrophe), or by a new process of endocycling, until surviving cells finally became apoptotic (96 h after the treatment). |
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Keywords: | Etoposide HeLa cells Metaphase arrest Mitotic catastrophe Apoptosis |
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