Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS |
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Authors: | Ming-Zhu Ding Jing-Sheng Cheng Wen-Hai Xiao Bin Qiao Ying-Jin Yuan |
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Affiliation: | (1) Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, P. O. Box 6888, Tianjin, 300072, People’s Republic of China |
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Abstract: | The intracellular metabolic profile characterization of Saccharomyces cerevisiae throughout industrial ethanol fermentation was investigated using gas chromatography coupled to time-of-flight mass spectrometry. A total of 143 and 128 intracellular metabolites in S. cerevisiae were detected and quantified in continuous and batch fermentations, respectively. The two fermentation processes were both clearly distinguished into three main phases by principal components analysis. Furthermore, the levels of some metabolites involved in central carbon metabolism varied significantly throughout both processes. Glycerol and phosphoric acid were principally responsible for discriminating seed, main and final phases of continuous fermentation, while lactic acid and glycerol contributed mostly to telling different phases of batch fermentation. In addition, the levels of some amino acids such as glycine varied significantly during both processes. These findings provide new insights into the metabolomic characteristics during industrial ethanol fermentation processes. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Metabolic profiles GC-TOF-MS Continuous fermentation Batch fermentation S. cerevisiae |
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