The role of RNA in the hormonal induction of tyrosine aminotransferase in mammalian cells in tissue culture |
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| Authors: | T D Gelehrter G M Tomkins |
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| Affiliation: | 1. TNO Energy Transition, the Netherlands;2. Faculty of Science and Engineering, University of Groningen, the Netherlands;1. Norwich Medical School, University of East Anglia, Norwich, UK;2. Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland;3. Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, OH, USA;4. Department of Surgery, Amsterdam Gastroenterology and Metabolism, Amsterdam Infection and Immunity, Amsterdam UMC, Location AMC, University of Amsterdam, the Netherlands;5. Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA;6. Armstrong Institute for Patient Safety and Quality, Johns Hopkins Medicine, Baltimore, MD, USA;7. Infection Prevention and Control Global Unit, Service Delivery and Safety, World Health Organization, Geneva, Switzerland;1. Thermal and Environmental Engineering Institute, Tongji University, 200092 Shanghai, China;2. Shanghai Municipal Engineering Design Institute (Group) Co., LTD, 200092 Shanghai, China |
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| Abstract: | Under conditions in which dexamethasone induced a 3- to 15-fold increase in tyrosine aminotransferase activity in hepatoma cells in tissue culture, there was no significant increase in total cellular RNA, nor in its rate of synthesis as measured by the rate of incorporation of labeled precursors. Furthermore, no hormonal effect on the turnover of rapidly labeled RNA was demonstrable after the administration of unlabeled uridine or actinomycin D. A variety of experiments failed to demonstrate the gross stimulation of RNA synthesis associated with enzyme induction by steroid hormones in vivo, suggesting that these changes are not an essential part of the mechanism of enzyme induction by glucocorticoids.Analysis of several subcellular RNA fractions by sucrose gradient and double-labeling techniques confirmed the absence of any gross stimulation of RNA synthesis by glucocorticoids. These studies also demonstrated that dexamethasone had no effect on the labeling pattern of cytoplasmic rRNA or transfer RNA. There was a suggestion that the hormone might stimulate the synthesis of a small amount of rapidly labeled cytoplasmic RNA associated with ribosomes, and sedimenting with the 45 s particle. |
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