Detection of microcystin-producing Microcystis in Guanqiao Lake using a sandwich hybridization assay |
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Authors: | Zhu Jing Ping Du Shi Li Xian |
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Affiliation: | School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China. |
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Abstract: | Based on sequence analyses of the mcyJ gene from Microcystis strains, a probe pair TJF and TJR was designed and a sandwich hybridization assay (SHA) was established to quantitatively detect microcystin-producing Microcystis. Through BLAST and cyanobacterial culture tests, TJF and TJR were demonstrated to be specific for microcystin-producing Microcystis. A calibration curve for the SHA was established, and the lowest detected concentration was 100 cells·mL(-1). Laboratory cultures and field samples from Guanqiao Lake were analyzed with both the SHA and microscopy. The cell number of microcystin-producing Microcystis and that of total Microcystis were compared. The biotic and abiotic components of the samples were of little disturbance to the SHA. In this study, a SHA was established to detect Microcystis, providing an alternative to PCR-ELISA and real-time PCR technology. |
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