Membrane topology and functional analysis of Methylobacillus sp. 12S genes epsF and epsG, encoding polysaccharide chain-length determining proteins |
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Authors: | Ayabe-Chujo Yuko Usami Yusuke Yoshida Takako Omori Toshio Nojiri Hideaki |
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Institution: | Biotechnology Research Center, The University of Tokyo, Tokyo, Japan. |
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Abstract: | The EpsF and EpsG of the methanol-assimilating bacterium Methylobacillus sp. 12S are involved in the synthesis of a high molecular weight exopolysaccharide, methanolan. These proteins share homology with chain-length determiners in other polysaccharide-producing bacteria. The N- and C-termini of EpsF were found to locate to the cytoplasm, and EpsF was predicted to have two transmembrane regions. EpsG showed both ATPase and autophosphorylation activities. |
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