抑制T细胞增殖的抗CD98重链单克隆抗体的建立

目的寻找能调节T细胞功能的相关分子,进行与T细胞介导的自身免疫性疾病相关的研究。方法收集BALB/c小鼠脾细胞,免疫Wistar大鼠,进行细胞融合,建立杂交瘤细胞系。筛选得到43株能调节T细胞功能的杂交瘤细胞系,对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果显示其目标分子是CD98重链,同时后续实验显示抗CD98单克隆抗体能抑制纤连蛋白介导的细胞分布,但不影响氨基酸转运。而且混合淋巴细胞反应显示该抗体能显著抑制T细胞增殖反应。结论抗CD98单克隆抗体能有效抑制T细胞增殖,有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。

Establishment of an anti-CD98hc monoclonal antibody that inhibits T-cell proliferation

Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media- ted autoimmune diseases. Methods We collected splenoeytes from BALB/c mice to immunize Wistar rats and to establish many hybridoma cell lines. Forty-three hyhridoma cell lines which could modulate T-cell functions were obtained. One of the 43 cell lines, most actively inhibiting T-cell proliferation, was further studied. Results The monoclonal antibody anti- CD98hc mAb recognized CD98 heavy chain, and suppressed fihronectin-mediated cell spreading hut not amino acid trans- port. Furthermore, the anti-CD98hc mAh significantly suppressed T-cell proliferation in mixed lymphocyte reaction. Con- clusions Our results indicate that the anti-CD98hc mAb can effectively suppress T-cell proliferation, and is promising to be used in the prevention and treatment of T-cell mediated autoimmune diseases in the future.