MST3在颞叶癫痫模型小鼠海马的表达变化

目的观察海人酸(kainic acid,KA)所致癫痫(epilepsy,EP)小鼠海马Ste20蛋白激酵素(MST3)表达水平的变化,探讨MST3在癫痫发病过程中的可能作用。方法选用成年雄性小鼠,并随机分成模型组和对照组。模型组小鼠侧脑室注射2μL(100 ng/μL)KA,分别于术后3、8、24 h收集动物标本以进行检测。使用RT-PCR和Western Blot测定MST3 mRNA含量和MST3蛋白动态表达变化,应用免疫组化观察MST3在海马的表达分布与特点。结果与正常对照组相比,模型组海马组织内MST3mRNA的表达随时间持续升高,24 h达到高峰;MST3的蛋白表达也表现出同样的动态升高趋势;术后3～24 h的模型组海马免疫组化检测显示,模型组MST3主要以海马齿状回、门回区、CA3区域表达增加为主,并且这些区域表达逐渐递增。结论随着时间的推移,MST3表达水平呈现逐渐增加趋势,可能与神经元损伤造成的凋亡之间有密切的关系,提示MST3可能在癫痫发病过程中起重要作用。

Changes of MST3 expression in the hippocampus of mice with temporal lobe epilepsy

Objective To investigate the expression of mammalian STE20-1ike protein kinase 3 （MST3） in the hippocampus of epileptic mice induced by kainic acid （KA） , and to explore the role of MST3 in pathogenesis of epilepsy. Methods Adult male mice were randomly divided into control and experimental groups. The experimental group was injec- ted with 2 μL （ 100 ng/μL） KA in the lateral cerebral ventricle and detected at 3,8,24 hours after injection. The levels of mRNA and protein were detected by RT-PCR and Western blot, respectively. The dynamic changes of MST3 in the hippo- campus were revealed by immunohistochemistry. Results Compared with the control group, both the levels of MST3 mR- NA and protein in hippocampus of the experimental group were increased gradually over time and reached a peak at 24 hours after KA injection. The results of immunohistochemistry showed that the MST3 expression in the hippoeampus of ex- perimental group was increased, mainly in the dentate gyrus and CA3 area. Conclusions The MST3 expression in epilep- tic mice increases gradually during the disease progression, and may he related with apoptosis in neurons. MST3 may play an important role in epilepsy pathogenesis.