Cloning an ipt gene from Agrobacterium tumefaciens: characterisation of cytokinins in derivative transgenic plant tissue |
| |
| Authors: | Marian J. McKenzie Paula E. Jameson Russell T. M. Poulter |
| |
| Affiliation: | (1) Botany Department, University of Otago, P.O. Box 56, Dunedin, New Zealand;(2) Biochemistry Department, University of Otago, P.O. Box 56, Dunedin, New Zealand;(3) Present address: Department of Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North, New Zealand |
| |
| Abstract: | The isopentenyl transferase gene was isolated from Agrobacterium tumefaciens AcH5 using polymerase chain reaction and transformed into Petunia and Kalanchoë using both A. tumefaciens and A. rhizogenes transformation systems. Morphological evidence and elevated endogenous cytokinin levels indicated that the PCR product was an active gene. Accurate quantification of the cytokinins was obtained by radioimmunoassay, following purification and separation of the free bases and ribosides by HPLC. Of the six cytokinins quantified, zeatin riboside and its stabilised dihydro-derivative, dihydrozeatin riboside, showed the greatest increases in the transformed Petunia tissue (up to 600-fold). The importance of measuring changes in individual cytokinins is discussed. |
| |
| Keywords: | ipt gene cytokinin Agrobacterium tumefaciens Agrobacterium rhizogenes polymerase chain reaction, radioimmunoassay |
| 本文献已被 SpringerLink 等数据库收录！ |
|
